NEW YORK (GenomeWeb News) – A new study is revealing the stickiness of transcription factors, and its authors said should remind researchers of both the wealth and limits of genome-wide approaches to understanding molecular interactions.
Researchers from the Lawrence Berkeley National Laboratory and the Santa Clara, California-based company Affymetrix found more than they bargained for while looking for genomic DNA associated with transcription factors involved in fruit fly development. The six proteins they tested bound extensively — several thousand places on the genome. But much of the binding fell outside of sites believed to influence gene expression.
The authors claim these results, published in PLoS Biology online today, highlight the importance of careful analysis of interaction data. “Our conclusions differ from those of other groups who have not distinguished between different levels of DNA binding in vivo using similar assays and have generally assumed that all detected binding is functional,” the authors wrote.
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Researchers from the Lawrence Berkeley National Laboratory and the Santa Clara, California-based company Affymetrix found more than they bargained for while looking for genomic DNA associated with transcription factors involved in fruit fly development. The six proteins they tested bound extensively — several thousand places on the genome. But much of the binding fell outside of sites believed to influence gene expression.
The authors claim these results, published in PLoS Biology online today, highlight the importance of careful analysis of interaction data. “Our conclusions differ from those of other groups who have not distinguished between different levels of DNA binding in vivo using similar assays and have generally assumed that all detected binding is functional,” the authors wrote.
Read More Article...
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